Industrial Test Systems, Inc.’s EZ Coliform Cult-MUG Test is US EPA compliant for presence/absence determination of total coliforms and E. coli in water. Each test consists of a sterilized, ready-to-use 120 mL bottle containing a pre-measured amount of growth media. Add the water sample, cap the bottle and incubate at 35°C (95°F) for 24 hours or 25°C (77°F) for 48 hours. Coliform presence causes samples to change from yellow to blue-green. E. coli contaminated samples glow a bright blue, fluorescent color when illuminated with a UV light (365 nm). Alternatively, addition of Kovac’s reagent will confirm E. coli presence by producing a red ring.www.sensafe.com(800) 861-9712